Metabolite turnover

Plants rely on a complex metabolism fine-tuned through interconnected feedback loops and characterized by balanced investments of resources into growth and defense. Brassica crops and the closely related model plant Arabidopsis allocate carbon, nitrogen, and sulfur to synthesize glucosinolates as their key defensive metabolites.

Glucosinolates are inert and non-toxic compounds, but their activation by myrosinases upon tissue disruption releases an unstable intermediate, which can rearrange into chemically different, biologically active breakdown products depending on the side-chain chemistry and the plant genotype.

Glucosinolates are, however, also degraded in the absence of an attacker. This turnover of glucosinolates in intact tissue does not only allow plants to dynamically regulate glucosinolate levels to optimize defense, but also to recycle sulfur and nitrogen for general metabolic processes. In contrast to our detailed knowledge on the glucosinolate biosynthesis and glucosinolate activation upon attack, the pathways for glucosinolate turnover in intact tissue remained completely elusive.

Glucosinolates serve multiple functions in metabolism and defense

Read more.....

Jeschke, V., Weber, K., Moore, S. & Burow, M. (2019) Coordination of glucosinolate biosynthesis and turnover under different nutrient conditions. Frontiers in Plant Science. DOI:10.3389/fpls.2019.01560.

Blažević, I., Montaut, S., Burcul, F., Olsen, C.E., Burow, M., Rollin, P., Agerbirk, N. (2019) Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants. Phytochemistry. DOI:10.1016/j.phytochem.2019.112100.

Daniel Vik, Namiko Mitarai, Nikolai Wulff, Barbara A. Halkier and Meike Burow (2018). Dynamic Modeling of Indole Glucosinolate Hydrolysis and Its Impact on Auxin Signaling. Front. Plant Sci.; Apr 26; 9. DOI:10.3389/fpls.2018.00550