Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation
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Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation. / Han, Xiaoyu; Gao, Chen; Liang, Buyou; Cui, Jingxuan; Xu, Qiyu; Schulz, Alexander; Liesche, Johannes.
In: Tree Physiology, Vol. 42, No. 3, 2022, p. 488-500.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation
AU - Han, Xiaoyu
AU - Gao, Chen
AU - Liang, Buyou
AU - Cui, Jingxuan
AU - Xu, Qiyu
AU - Schulz, Alexander
AU - Liesche, Johannes
PY - 2022
Y1 - 2022
N2 - Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species. Here, we cloned two types of SUTs from two gymnosperms, the conifers Cedrus deodara (Roxb. G. Don) and Pinus massoniana Lambert, and analyzed their sucrose transport activities. Uptake of the fluorescent sucrose-analog esculin into tobacco epidermis cells expressing the conifer SUT confirmed their transport ability. To determine their function in planta, we investigated their mRNA abundance in relation to photosynthesis and sugar levels in leaves, inner bark, wood and roots. Combined with measurements of protein abundance and immunolocalization of C. deodara SUTs, our results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions. The implications of these findings regarding conifer physiology and SUT evolution are discussed.
AB - Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species. Here, we cloned two types of SUTs from two gymnosperms, the conifers Cedrus deodara (Roxb. G. Don) and Pinus massoniana Lambert, and analyzed their sucrose transport activities. Uptake of the fluorescent sucrose-analog esculin into tobacco epidermis cells expressing the conifer SUT confirmed their transport ability. To determine their function in planta, we investigated their mRNA abundance in relation to photosynthesis and sugar levels in leaves, inner bark, wood and roots. Combined with measurements of protein abundance and immunolocalization of C. deodara SUTs, our results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions. The implications of these findings regarding conifer physiology and SUT evolution are discussed.
U2 - 10.1093/treephys/tpab149
DO - 10.1093/treephys/tpab149
M3 - Journal article
C2 - 35020944
VL - 42
SP - 488
EP - 500
JO - Tree Physiology
JF - Tree Physiology
SN - 0829-318X
IS - 3
ER -
ID: 289910258