PhD Defence: Nadia M. A. Mirza
PhD Candidate
Nadia M. A. Mirza
Title of PhD Thesis
Engineering and Optimization of the Chain Elongation Pathway of Glucosinolate Biosynthesis
Abstract
Glucoraphanin is a health promoting secondary metabolite found in broccoli, it exhibits anti-cancer and antimicrobial properties.The thesis deals with metabolic engineering of glucoraphanin in heterologous systems. In addition, a minor part of the thesis describes the characterization of an antibody against glucoraphanin. The research conducted contributes mainly towards investigation and optimization of heterologous expression of the chain elongation pathway for glucoraphanin biosynthesis. Production of the glucoraphanin precursor in E. coli provides a proof-of-concept for transfer of chain elongation enzymes to E. coli cytosol and is an important step forward towards microbial production of glucoraphanin.
Supervisor
Professor Barbara Ann Halkier
Assessment Committee
Jing-Ke Weng, Dept. of Biology, MIT
Michael Dalgaard Mikkelsen, EVOLVA
Fernando Geu-Flores, University of Copenhagen (Chair)
Reception
The defence will be followed by a reception at Thorvaldsensvej 40, room R660 (6th floor at elevator 8)
