PhD and MSc theses from DynaMo Center

Novel molecular tools for crop development and synthesis of natural products - understanding glucosinolate based defenses in cruciferous plants.
Supervisor: Barbara Ann Halkier

Pathway elucidation and metabolic engineering of plant defense compounds for crop protection - case studies using camalexin and glucosinolates.
Supervisor: Barbara Ann Halkier

Towards sustainable potato production using biotechnology.
Supervisor: Barbara Ann Halkier

Master theses

Investigating mechanism involved in establishment of Arabidobsis glucosinolate  seed profile.

Development of Brassica napus seed meal suitable for human consumption

Characterization of a putative plastidic sugar transporter from Chlamydomonas reinhardtii and investigation of its ability to enhance mixotrophic growth on glucose

Evaluating the potential of beneficial bacterial strains as plant bio-inoculants

Off-flavours in rapeseed press cake. Investigation of 3-O-(2´´´-O-sinapoyl-β-sophoroside) accumulation in Brassica napus seeds

Transcriptomics to Study Transgenerational Resistance in Arabidopsis
Supervisors: Meike Burow & Emma Aller

Optimizing Glucoraphanin Production in Escherichia coli by Genome Integration
Supervisors: Barbara Ann Halkier & Michal Poborsky

Synthetic biology approach to build yeast cell factory producing health-promoting
glucosinolates
Supervisors: Barbara Ann Halkier & William Thomas Wajn

Characterizing ABCC6 substrate specificity – A long-sought key to prevent vascular calcification
Supervisors: Hussam Nour-Eldin &Henrik Dimke (University of Southern Denmark)

Characterization of a putative tZ transporter DTX18 and its close homolog
Supervisors: Hussam Nour-Eldin & Zeinu Mussa-Belew

Elucidating the amino acid residues responsible for the substrate specificity of glucosinolate exporters
Supervisors: Barbara Ann Halkier & Deyang Xu

Screening of Environmentally Diverse Soils to Discover New Beneficial Microbes for Use in Sustainable Agriculture.
Supervisors: Barbara Ann Halkier, Deyang Xu & Laura Dethier

Harnessing beneficial microbes from Danish nature
Supervisors: Barbara Ann Halkier & Laura Dethier

Transgenerational Plant Defense against Spider Mite
Attack in Arabidopsis thaliana
Supervisor: Meike Burow & Cristina Rioja

Trophic Conversion of Chlamydomonas reinhardtii via Functional Expression of
Passive Glucose Transporters
Supervisor: Hussam Nour-Eldin & Antonina Karakostova

Characterising an Unknown Sugar. Transporter in the Genome of Chlamydomonas reinhardtii
Supervisor: Hussam Nour-Eldin & Antonina Karakostova

Identification of Glucosinolate vacuolar transporters
Supervisor: Barbara Ann Halkier & Deyang Xu

Generating and screening GTR2 transporter mutant libraries for detection of hyperactive
mutants
Supervisor: Hussam Nour-Eldin & Christa Kanstrup

Glucosinolate biosynthesis in yeast
Supervisor: Barbara Ann Halkier

Engineering synthetic recursive amino acid chain elongation pathway in E. coli
Supervisor: Barbara Ann Halkier & Michal Poborsky

Identifyding pathogen genes involved in plant host infection.
Utilizing the Arabidopsis-Botrytis interaction for identification of candidate genes
Supervisor: Meike Burow

Uncaging glycolysis in C hlamydomonas reinhardtii to enable growth on external glucose
Supervisor: Hussam Nour-Eldin & Antonina Karakostova

From growth-inhibiting to healthy: Eliminating progoitrin and enriching glucoraphanin
through CRISPR-induced mutagenesis in A. thaliana and B. napus
Supervisor: Barbara Ann Halkier and Deyang Xu

New insights into the relation between the glucosinolate transcription factors and the ROS related SRO family in Arabidopsis thaliana
Supervisor: Meike Burow & Henrik Frisenvang

Jakob Harken Ellefsen

Exploring protein-protein interactions of intrinsically disordered proteins
Supervisor: Meike Burow

Ling Chen

Optimization of glucosinolate production in Saccharomyces cerevisiae
Supervisor: Barbara Ann Halkier & Cuiwei Wang

A novel untargeted extract-based approach to unravel transporters' substrate spectra
Supervisor: Hussam Nour-Eldin

Metabolite profiling of plants grown with beneficial bacteria
Supervisor: Meike Burow

Gain-of-Function Transporters - Development of a screening system for high-throughput identification and application in transport engineering of specialized metabolites
Supervisor: Hussam Nour-Eldin & Nikolaj Wulff

Towards sugar-based growth of Chlamydomonas reinhardtii
Supervisor: Hussam Nour-Eldin

Non-invasive bioimaging of RNA-protein interactions
Supervisor: Meike Burow

Marie Louise Fobian Thomsen

Natural Variation in Root Growth Response to 3ohp
Glucosinolate: Utilizing Natural Variation for Identification of Functional Targets of 3ohp
Supervisor: Meike Burow & Frederikke Malinovsky

Christian Arentz Sørensen

Increasing the rate of homologous recombination in C. reinhardtii
Supervisor: Hussam Nour-Eldin

High-throughput identification of transporters for plant specialized metabolites
Supervisor: Hussam Nour-Eldin

Microbial production of glucosinolates using synthetic biology
Supervisor: Barbara Ann Halkier

Epigenetic regulation of plant defence: assessing the impact of DNA methylations on glucosinolates
Supervisor: Meike Burow

Structure-function relationship of MYB transcription factors in Arabidopsis thaliana
Supervisor: Meike Burow

Genetic Engineering of Chlamydomonas reinhardtii - Glucosinolate production in green microalgae
Supervisor: Hussam Nour-Eldin

Protein and metabolic engineering for microbial production of glucoraphanin
Supervisor: Barbara Ann Halkier

Towards rationally engineering specialized metabolite transport in plants
Supervisor: Hussam Nour-Eldin

Identification and characterization of plant transporters from the model plant Arabidopsis thaliana
Supervisor: Dietmar Geiger (U of Würzburg) & Hussam Nour-Eldin

Investigations of the putative glucosinolate metabolon
Supervisor: Barbara Ann Halkier

Regulation of glucosinolate transport: Effects of phosphorylation on AtGTR1 and AtGTR2 transport activity
Supervisor: Barbara Ann Halkier & Hussam Nour-Eldin