Differential partitioning of thiols and glucosinolates between shoot and root in Chinese cabbage upon excess zinc exposure
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Differential partitioning of thiols and glucosinolates between shoot and root in Chinese cabbage upon excess zinc exposure. / Aghajanzadeh, Tahereh A.; Prajapati, Dharmendra H.; Burow, Meike.
In: Journal of Plant Physiology, Vol. 244, 153088, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Differential partitioning of thiols and glucosinolates between shoot and root in Chinese cabbage upon excess zinc exposure
AU - Aghajanzadeh, Tahereh A.
AU - Prajapati, Dharmendra H.
AU - Burow, Meike
PY - 2020
Y1 - 2020
N2 - Zinc (Zn) is one of the important elements of plant growth, however, at elevated level it is toxic. Exposure of Chinese cabbage to elevated Zn2+ concentrations (5 and 10 mu M ZnCl2) resulted in enhancement of total sulfur and organic sulfur concentration. Transcript level of APS reductase (APR) as a key enzyme in biosynthesis of primary sulfur compounds (cysteine and thiols), was up-regulated in both shoot and root upon exposure to elevated Zn2+, which was accompanied by an increase in the concentration of cysteine in both tissues. In contrast, the concentration of thiols increased only in the root by 5.5 and 15-fold at 5 and 10 mu M Zn2+, respectively, which was in accompanied by an upregulation of ATP sulfurylase, an enzyme responsible for activation of sulfate. An elevated content of glucosinolates, mostly indolic glucosinolates, only in the shoot of plants exposed to excess level of Zn2+ coincided with an increase in gene expression of key biosynthetic enzymes and regulators (CYP79B3, CYP83B1, MYB34). Thus distinct acuumulation patterns of sulfur containing compounds in root and shoot of Chinese cabbage may be a strategy for Chinese cabbage to combat with exposure to excess Zn.
AB - Zinc (Zn) is one of the important elements of plant growth, however, at elevated level it is toxic. Exposure of Chinese cabbage to elevated Zn2+ concentrations (5 and 10 mu M ZnCl2) resulted in enhancement of total sulfur and organic sulfur concentration. Transcript level of APS reductase (APR) as a key enzyme in biosynthesis of primary sulfur compounds (cysteine and thiols), was up-regulated in both shoot and root upon exposure to elevated Zn2+, which was accompanied by an increase in the concentration of cysteine in both tissues. In contrast, the concentration of thiols increased only in the root by 5.5 and 15-fold at 5 and 10 mu M Zn2+, respectively, which was in accompanied by an upregulation of ATP sulfurylase, an enzyme responsible for activation of sulfate. An elevated content of glucosinolates, mostly indolic glucosinolates, only in the shoot of plants exposed to excess level of Zn2+ coincided with an increase in gene expression of key biosynthetic enzymes and regulators (CYP79B3, CYP83B1, MYB34). Thus distinct acuumulation patterns of sulfur containing compounds in root and shoot of Chinese cabbage may be a strategy for Chinese cabbage to combat with exposure to excess Zn.
KW - Chinese cabbage
KW - Glucosinolates
KW - Thiols
KW - Sulfur metabolism
KW - Zinc
KW - ARABIDOPSIS-THALIANA
KW - ATP SULFURYLASE
KW - BRASSICA-OLERACEA
KW - PHYSIOLOGICAL FUNCTIONS
KW - SECONDARY METABOLITES
KW - TRANSCRIPTION FACTORS
KW - SULFATE TRANSPORT
KW - ASSIMILATION
KW - CADMIUM
KW - BIOSYNTHESIS
U2 - 10.1016/j.jplph.2019.153088
DO - 10.1016/j.jplph.2019.153088
M3 - Journal article
C2 - 31812906
VL - 244
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
M1 - 153088
ER -
ID: 249486036