Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling. / Hong, Shin-Young; Sun, Bin; Straub, Daniel; Blaakmeer, Anko; Mineri, Lorenzo; Koch, Jonas; Brinch-Pedersen, Henrik; Holme, Inger B.; Burow, Meike; Jørgensen, Hans Jørgen Lyngs; Mar Alba, M.; Wenkel, Stephan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, No. 42, 2020, p. 26197-26205.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hong, S-Y, Sun, B, Straub, D, Blaakmeer, A, Mineri, L, Koch, J, Brinch-Pedersen, H, Holme, IB, Burow, M, Jørgensen, HJL, Mar Alba, M & Wenkel, S 2020, 'Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 42, pp. 26197-26205. https://doi.org/10.1073/pnas.2005198117

APA

Hong, S-Y., Sun, B., Straub, D., Blaakmeer, A., Mineri, L., Koch, J., Brinch-Pedersen, H., Holme, I. B., Burow, M., Jørgensen, H. J. L., Mar Alba, M., & Wenkel, S. (2020). Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling. Proceedings of the National Academy of Sciences of the United States of America, 117(42), 26197-26205. https://doi.org/10.1073/pnas.2005198117

Vancouver

Hong S-Y, Sun B, Straub D, Blaakmeer A, Mineri L, Koch J et al. Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling. Proceedings of the National Academy of Sciences of the United States of America. 2020;117(42):26197-26205. https://doi.org/10.1073/pnas.2005198117

Author

Hong, Shin-Young ; Sun, Bin ; Straub, Daniel ; Blaakmeer, Anko ; Mineri, Lorenzo ; Koch, Jonas ; Brinch-Pedersen, Henrik ; Holme, Inger B. ; Burow, Meike ; Jørgensen, Hans Jørgen Lyngs ; Mar Alba, M. ; Wenkel, Stephan. / Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling. In: Proceedings of the National Academy of Sciences of the United States of America. 2020 ; Vol. 117, No. 42. pp. 26197-26205.

Bibtex

@article{dd2e5c313391485ba9d8b7ceea343842,
title = "Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling",
abstract = "MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD. Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures.",
keywords = "microProteins, jasmonic acid, branching, CELL ELONGATION, MISSING LINK, ARABIDOPSIS, TOPLESS, PROTEIN, RICE, DWARF, GENE, SIZE",
author = "Shin-Young Hong and Bin Sun and Daniel Straub and Anko Blaakmeer and Lorenzo Mineri and Jonas Koch and Henrik Brinch-Pedersen and Holme, {Inger B.} and Meike Burow and J{\o}rgensen, {Hans J{\o}rgen Lyngs} and {Mar Alba}, M. and Stephan Wenkel",
year = "2020",
doi = "10.1073/pnas.2005198117",
language = "English",
volume = "117",
pages = "26197--26205",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "42",

}

RIS

TY - JOUR

T1 - Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling

AU - Hong, Shin-Young

AU - Sun, Bin

AU - Straub, Daniel

AU - Blaakmeer, Anko

AU - Mineri, Lorenzo

AU - Koch, Jonas

AU - Brinch-Pedersen, Henrik

AU - Holme, Inger B.

AU - Burow, Meike

AU - Jørgensen, Hans Jørgen Lyngs

AU - Mar Alba, M.

AU - Wenkel, Stephan

PY - 2020

Y1 - 2020

N2 - MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD. Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures.

AB - MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD. Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures.

KW - microProteins

KW - jasmonic acid

KW - branching

KW - CELL ELONGATION

KW - MISSING LINK

KW - ARABIDOPSIS

KW - TOPLESS

KW - PROTEIN

KW - RICE

KW - DWARF

KW - GENE

KW - SIZE

U2 - 10.1073/pnas.2005198117

DO - 10.1073/pnas.2005198117

M3 - Journal article

C2 - 33033229

VL - 117

SP - 26197

EP - 26205

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 42

ER -

ID: 251025102