Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

Research output: Contribution to journal › Letter › Research › peer-review

Standard

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis. / Tiukova, Ievgeniia A.; Møller-Hansen, Iben; Belew, Zeinu M.; Darbani, Behrooz; Boles, Eckhard; Nour-Eldin, Hussam H.; Linder, Tomas; Nielsen, Jens; Borodina, Irina.

In: FEMS Microbiology Letters, Vol. 366, No. 17, fnz222, 01.09.2019, p. 1-9.

Research output: Contribution to journal › Letter › Research › peer-review

Harvard

Tiukova, IA, Møller-Hansen, I, Belew, ZM, Darbani, B, Boles, E, Nour-Eldin, HH, Linder, T, Nielsen, J & Borodina, I 2019, 'Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis', FEMS Microbiology Letters, vol. 366, no. 17, fnz222, pp. 1-9. https://doi.org/10.1093/femsle/fnz222

APA

Tiukova, I. A., Møller-Hansen, I., Belew, Z. M., Darbani, B., Boles, E., Nour-Eldin, H. H., Linder, T., Nielsen, J., & Borodina, I. (2019). Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis. FEMS Microbiology Letters, 366(17), 1-9. [fnz222]. https://doi.org/10.1093/femsle/fnz222

Vancouver

Tiukova IA, Møller-Hansen I, Belew ZM, Darbani B, Boles E, Nour-Eldin HH et al. Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis. FEMS Microbiology Letters. 2019 Sep 1;366(17):1-9. fnz222. https://doi.org/10.1093/femsle/fnz222

Author

Tiukova, Ievgeniia A. ; Møller-Hansen, Iben ; Belew, Zeinu M. ; Darbani, Behrooz ; Boles, Eckhard ; Nour-Eldin, Hussam H. ; Linder, Tomas ; Nielsen, Jens ; Borodina, Irina. / Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis. In: FEMS Microbiology Letters. 2019 ; Vol. 366, No. 17. pp. 1-9.

Bibtex

@article{dfc06a56931b4b67a0e4b8db92931d52,
title = "Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis",
abstract = "The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.",
keywords = "Brettanomyces bruxellensis, Xenopus laevis, bioethanol, glucose transport, high-affinity, metabolism, yeast",
author = "Tiukova, {Ievgeniia A.} and Iben M{\o}ller-Hansen and Belew, {Zeinu M.} and Behrooz Darbani and Eckhard Boles and Nour-Eldin, {Hussam H.} and Tomas Linder and Jens Nielsen and Irina Borodina",
year = "2019",
month = sep,
day = "1",
doi = "10.1093/femsle/fnz222",
language = "English",
volume = "366",
pages = "1--9",
journal = "F E M S Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "17",

}

RIS

TY - JOUR

T1 - Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

AU - Tiukova, Ievgeniia A.

AU - Møller-Hansen, Iben

AU - Belew, Zeinu M.

AU - Darbani, Behrooz

AU - Boles, Eckhard

AU - Nour-Eldin, Hussam H.

AU - Linder, Tomas

AU - Nielsen, Jens

AU - Borodina, Irina

PY - 2019/9/1

Y1 - 2019/9/1

N2 - The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

AB - The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

KW - Brettanomyces bruxellensis

KW - Xenopus laevis

KW - bioethanol

KW - glucose transport

KW - high-affinity

KW - metabolism

KW - yeast

U2 - 10.1093/femsle/fnz222

DO - 10.1093/femsle/fnz222

M3 - Letter

C2 - 31665273

VL - 366

SP - 1

EP - 9

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 17

M1 - fnz222

ER -

ID: 234142726