The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness. / Kazachkova, Yana; Zemach, Itay; Panda, Sayantan; Bocobza, Samuel; Vainer, Andrii; Rogachev, Ilana; Dong, Yonghui; Ben-Dor, Shifra; Veres, Dorottya; Kanstrup, Christa; Lambertz, Sophie Konstanze; Crocoll, Christoph; Hu, Yangjie; Shani, Eilon; Michaeli, Simon; Nour-Eldin, Hussam Hassan; Zamir, Dani; Aharoni, Asaph.

In: Nature Plants, Vol. 7, 2021, p. 468-480.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kazachkova, Y, Zemach, I, Panda, S, Bocobza, S, Vainer, A, Rogachev, I, Dong, Y, Ben-Dor, S, Veres, D, Kanstrup, C, Lambertz, SK, Crocoll, C, Hu, Y, Shani, E, Michaeli, S, Nour-Eldin, HH, Zamir, D & Aharoni, A 2021, 'The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness', Nature Plants, vol. 7, pp. 468-480. https://doi.org/10.1038/s41477-021-00865-6

APA

Kazachkova, Y., Zemach, I., Panda, S., Bocobza, S., Vainer, A., Rogachev, I., Dong, Y., Ben-Dor, S., Veres, D., Kanstrup, C., Lambertz, S. K., Crocoll, C., Hu, Y., Shani, E., Michaeli, S., Nour-Eldin, H. H., Zamir, D., & Aharoni, A. (2021). The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness. Nature Plants, 7, 468-480. https://doi.org/10.1038/s41477-021-00865-6

Vancouver

Kazachkova Y, Zemach I, Panda S, Bocobza S, Vainer A, Rogachev I et al. The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness. Nature Plants. 2021;7:468-480. https://doi.org/10.1038/s41477-021-00865-6

Author

Kazachkova, Yana ; Zemach, Itay ; Panda, Sayantan ; Bocobza, Samuel ; Vainer, Andrii ; Rogachev, Ilana ; Dong, Yonghui ; Ben-Dor, Shifra ; Veres, Dorottya ; Kanstrup, Christa ; Lambertz, Sophie Konstanze ; Crocoll, Christoph ; Hu, Yangjie ; Shani, Eilon ; Michaeli, Simon ; Nour-Eldin, Hussam Hassan ; Zamir, Dani ; Aharoni, Asaph. / The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness. In: Nature Plants. 2021 ; Vol. 7. pp. 468-480.

Bibtex

@article{99f2eb1e1ae4494d87b3ca511640724d,
title = "The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness",
abstract = "Fruit taste is determined by sugars, acids and in some species, bitter chemicals. Attraction of seed-dispersing organisms in nature and breeding for consumer preferences requires reduced fruit bitterness. A key metabolic shift during ripening prevents tomato fruit bitterness by eliminating α-tomatine, a renowned defence-associated Solanum alkaloid. Here, we combined fine mapping with information from 150 resequenced genomes and genotyping a 650-tomato core collection to identify nine bitter-tasting accessions including the {\textquoteleft}high tomatine{\textquoteright} Peruvian landraces reported in the literature. These {\textquoteleft}bitter{\textquoteright} accessions contain a deletion in GORKY, a nitrate/peptide family transporter mediating α-tomatine subcellular localization during fruit ripening. GORKY exports α-tomatine and its derivatives from the vacuole to the cytosol and this facilitates the conversion of the entire α-tomatine pool to non-bitter forms, rendering the fruit palatable. Hence, GORKY activity was a notable innovation in the process of tomato fruit domestication and breeding.",
author = "Yana Kazachkova and Itay Zemach and Sayantan Panda and Samuel Bocobza and Andrii Vainer and Ilana Rogachev and Yonghui Dong and Shifra Ben-Dor and Dorottya Veres and Christa Kanstrup and Lambertz, {Sophie Konstanze} and Christoph Crocoll and Yangjie Hu and Eilon Shani and Simon Michaeli and Nour-Eldin, {Hussam Hassan} and Dani Zamir and Asaph Aharoni",
year = "2021",
doi = "10.1038/s41477-021-00865-6",
language = "English",
volume = "7",
pages = "468--480",
journal = "Nature Plants",
issn = "2055-026X",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness

AU - Kazachkova, Yana

AU - Zemach, Itay

AU - Panda, Sayantan

AU - Bocobza, Samuel

AU - Vainer, Andrii

AU - Rogachev, Ilana

AU - Dong, Yonghui

AU - Ben-Dor, Shifra

AU - Veres, Dorottya

AU - Kanstrup, Christa

AU - Lambertz, Sophie Konstanze

AU - Crocoll, Christoph

AU - Hu, Yangjie

AU - Shani, Eilon

AU - Michaeli, Simon

AU - Nour-Eldin, Hussam Hassan

AU - Zamir, Dani

AU - Aharoni, Asaph

PY - 2021

Y1 - 2021

N2 - Fruit taste is determined by sugars, acids and in some species, bitter chemicals. Attraction of seed-dispersing organisms in nature and breeding for consumer preferences requires reduced fruit bitterness. A key metabolic shift during ripening prevents tomato fruit bitterness by eliminating α-tomatine, a renowned defence-associated Solanum alkaloid. Here, we combined fine mapping with information from 150 resequenced genomes and genotyping a 650-tomato core collection to identify nine bitter-tasting accessions including the ‘high tomatine’ Peruvian landraces reported in the literature. These ‘bitter’ accessions contain a deletion in GORKY, a nitrate/peptide family transporter mediating α-tomatine subcellular localization during fruit ripening. GORKY exports α-tomatine and its derivatives from the vacuole to the cytosol and this facilitates the conversion of the entire α-tomatine pool to non-bitter forms, rendering the fruit palatable. Hence, GORKY activity was a notable innovation in the process of tomato fruit domestication and breeding.

AB - Fruit taste is determined by sugars, acids and in some species, bitter chemicals. Attraction of seed-dispersing organisms in nature and breeding for consumer preferences requires reduced fruit bitterness. A key metabolic shift during ripening prevents tomato fruit bitterness by eliminating α-tomatine, a renowned defence-associated Solanum alkaloid. Here, we combined fine mapping with information from 150 resequenced genomes and genotyping a 650-tomato core collection to identify nine bitter-tasting accessions including the ‘high tomatine’ Peruvian landraces reported in the literature. These ‘bitter’ accessions contain a deletion in GORKY, a nitrate/peptide family transporter mediating α-tomatine subcellular localization during fruit ripening. GORKY exports α-tomatine and its derivatives from the vacuole to the cytosol and this facilitates the conversion of the entire α-tomatine pool to non-bitter forms, rendering the fruit palatable. Hence, GORKY activity was a notable innovation in the process of tomato fruit domestication and breeding.

U2 - 10.1038/s41477-021-00865-6

DO - 10.1038/s41477-021-00865-6

M3 - Journal article

C2 - 33707737

AN - SCOPUS:85102503502

VL - 7

SP - 468

EP - 480

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

ER -

ID: 259815227