Hormone transport in plants
Unlike animals, plants are sessile organisms and must integrate biotic and abiotic stimuli for continuous growth and development throughout their lifespan. For example, plants determine the timing and location of growth and development depending on the availability and quality of nutrients and water, herbivory and disease state. To make these signal integration processes, plants employ phytohormone response pathways, which are controlled at multiple levels including biosynthesis, metabolism, perception and signaling. In addition, plants also regulate the distribution and concentration gradients of phytohormones, enabling highly coordinated cellular responses. For example, the combined activity of auxin influx and efflux carrier proteins generates local auxin maxima and directional gradients that inform essential developmental patterning. Similarly, hormone radiolabeling and mutant grafting experiments indicate that jasmonic acid, abscisic acid and gibberellic acid are mobile phytohormones and that their movement is important for transmitting critical information such as wounding and drought and for coordinating growth.
Using a functional genomics approach we are currently focusing on identifying and characterizing transporter complements for JA, ABA and GA. We have built a comprehensive library of membrane transporters from Arabidopsis thaliana and use the Xenopus oocyte system for screening and biochemical characterization of new transporters using electrophysiology and LCMS based uptake assays. We aim to elucidate the mechanism and physiological role of phytohormone movement in plants, which we expect will lead to the development of new strategies for improving agricultural traits.
Eilon Shani, Tel Aviv University, Israel
Toshimitsu Kawate, Cornell University, USA
Human Frontier Science Program extention (HFSP) 2018 - 2024
Human Frontier Science Program (HFSP) 2015-2018
Volodymyr Radchuk, Zeinu M. Belew, Andre Gündel, Simon Mayer, Alexander Hilo, Goetz Hensel Rajiv Sharma, Kerstin Neumann, Stefan Ortleb, Steffen Wagner, Aleksandra Muszynska, Christoph Crocoll, Deyang Xu, Iris Hoffie, Jochen Kumlehn, Joerg Fuchs, Fritz F. Peleke, Jedrzej J. Szymanski, Hardy Rolletschek, Hussam H. Nour-Eldin and Ljudmilla Borisjuk. ”HvSWEET11b transports both sugar and cytokinin in the developing barley grain” (2023) The Plant Cell, accepted for publication in proofs
Shani, Eilon, Jenia Binenbaum, Nikolai Wulff, Lucie Camut, Kristian Kiradjiev, Iris Tal, Himabindu Vasuki et al. "Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis." Preprint (2022) DOI: 10.21203/rs.3.rs-1670556/v1 – co-corresponding author, in final revision at Nature Plants
Yuqin Zhang, Himabindu Vasuki, Jie Liu, Hamutal Bar, Shani Lazary, Aiman Egbaria, Dagmar Ripper, Laurence Charrier, Zeinu Mussa, Nikolai Wulff, Hussam H. Nour-Eldin, Asaph Aharoni, Laura Ragni, Lucia Strader, Nir Sade, Roy Weinstain, Markus Geisler, Eilon Shani"ABA homeostasis and long-distance translocation is redundantly regulated by ABCG ABA importers."
Science advances 7 no. 43 (2021): eabf6069. DOI: 10.1126/sciadv.abf6069
Wulff, Nikolai, Heidi Asschenfeldt Ernst, Morten Egevang Jørgensen, Sophie Lambertz, Tobias Maierhofer, Zeinu Mussa Belew, Christoph Crocoll, Mohammed Saddik Motawia, Dietmar Geiger, Flemming Steen Jørgensen, Osman Mirza and Hussam Hassan Nour-Eldin. "An Optimized Screen Reduces the Number of GA Transporters and provides Insights into NPF Substrate Determinants." Frontiers in plant science 10 (2019): 1106. https://doi.org/10.3389/fpls.2019.01106
Zhang, Yuqin, Victoria Nasser, Odelia Pisanty, Moutasem Omary, Nikolai Wulff , Martin Di Donato,Iris Tal, Julian I. Schroeder, Markus Geisler, Hussam Hassan Nour-Eldin and Eilon Shani “A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport” Nature communications 9 (2018): 4204. DOI:10.1038/s41467-018-06410
Tal I, Zhang Y, Jørgensen ME, Pisanty O, Barbosa ICR, Zourelidou M, Regnault T, Crocoll C, Olsen CE, Weinstain R, Schwechheimer C, Halkier BA, Nour-Eldin HH, Estelle M, Shani E (2016) The Arabidopsis NPF3 protein is a GA transporter. Nat Commun 7: 11486. DOI: 10.1038/ncomms11486