Identification of the glucosinolate transporter complement
Intra- and intercellular transport of metabolites is essential for many processes, such as nutrient acquisition, cellular homeostasis, cell communication, and for coordination of growth and defense responses in multi-cellular organisms. Moreover, upon long-distance transport, a given metabolite has to cross multiple membrane barriers as it exits source tissues and enters sink tissues. At the subcellular level, additional barriers are crossed due to compartmentalization.
Arabidopsis with its ‘omics tools and mutant collection and mobile glucosinolate defence compounds is a powerful model system to study general transport processes in plants. In contrast to mutating transport of primary metabolites, perturbing the glucosinolate transport pathway does not result in major pleiotropic effects. Thus, by identifying the full transporter complement of glucosinolates, will enable us to characterize all critical barriers along the long-distance transport pathway of a metabolite in a multi-cellular organism.
We have identified two glucosinolate importers which are essential for seed accumulation, in intra- and interleaf distribution and for exudation into the rhizosphere, where glucosinolates play important role in shaping the microbial community in the rhizosphere.
However, we still do not know how glucosinolates are exported into the apoplast from biosynthetic domains and what the physiological importance is of these export processes. Moreover, we know that glucosinolates are stored in vacuoles but are made in the cytosol. How are glucosinolates moved into the vacuole? And are they remobilized for long distance transport to other tissues during development? These questions can only be answered by identifying the full glucosinolate transporter complement at the molecular level.
We will identify vacuolar importers and exporters of glucosinolates by screening for transport activity using cDNA libraries expressed in oocytes of Xenopus laevis and by searching in co-expression databases. Candidate transporters will be validated in planta using loss-of-function mutants and grafting experiments with GLS biosynthesis mutants and transporter mutants.
Ultimately, we expect to unravel how glucosinolates are translocated from production site to storage site, and to generate a dynamic model for movement of these compounds within and between organs in the plant throughout development.
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Xu D, Hunziker P, Koroleva O, Blennow A, Crocoll C, Schulz A, Nour-Eldin HH, Halkier BA (2019 GTR-mediated radial import directs accumulation of defensive glucosinolates to sulfur-rich cells (S-cells) in phloem cap of inflorescence stem of Arabidopsis thaliana. Mol Plant. DOI: 10.1016/j.molp.2019.06.008
Hunziker P, Halkier BA, Schulz A (2019) Arabidopsis glucosinolate storage cells transform into phloem fibres at late stages of development. J Exp Bot. DOI:10.1093/jxb/erz176
Larsen B, Xu D, Halkier BA, Nour-Eldin HH (2017) Advances in methods for identification and characterization of plant transporter function. J Exp Bot.68, 4045-4056. DOI: 10.1093/jxb/erx140
Nour-Eldin HH, Madsen SR, Engelen S, Jørgensen ME, Olsen CE, Andersen JS, Seynnaeve D, Verhoye T, Fulawka R, Denolf P, Halkier BA (2017) Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. Nat Biotechnol. DOI: 10.1038/nbt.3823
Jørgensen ME, Nour-Eldin HH, Halkier BA (2015) Transport of defense compounds from source to sink: lessons learned from glucosinolates. Trends Plant Sci 20: 508–514. DOI: 10.1016/j.tplants.2015.04.006
Madsen SR, Olsen CE, Nour-Eldin HH, Halkier BA (2014) Elucidating the role of transport processes in leaf glucosinolate distribution. Plant Physiol 166: 1450–1462. DOI: 10.1104/pp.114.246249
Andersen TG, Nour-Eldin HH, Fuller VL, Olsen CE, Burow M, Halkier BA (2013) Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis. Plant Cell 25: 3133–3145. DOI: 10.1105/tpc.113.110890
Nour-Eldin HH, Andersen TG, Burow M, Madsen SR, Jørgensen ME, Olsen CE, Dreyer I, Hedrich R, Geiger D, Halkier BA (2012) NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds. Nature 488: 531–534. DOI: 10.1093/gbe/evs063