Glucosinolates and Arabidopsis

Identification of the glucosinolate transporter complement

Glucosinolates and Arabidopsis is an excellent model system to study the dynamic molecular interactions between components of the biosynthetic machinery distributed across several compartments, and to study how glucosinolates are translocated from production to storage site.

Intra- and intercellular transport of metabolites is essential for many processes, such as cellular homeostasis, cell communication with the surroundings and coordination of growth and defense responses in plants. Using the glucosinolate defense compounds in the model plant Arabidopsis thaliana, we aim to identify the first full transporter complement of given metabolites. This includes characterization of the multiple membrane barriers of the transport pathway – from when glucosinolates exit source tissues to they reach their destination.

Today, we have identified the plasmamembrane-localized glucosinolate importers, the GTRs,  and exporters, UMAMITs, which are essential for intercellular distribution and seed accumulation. However, we still do not know how glucosinolates are glucosinolates are moved into the vacuole, exported out of epidermis, and whether they are remobilized to other tissues during development?

We will identify additional glucosinolate transporters by screening for transport activity using cDNA libraries expressed in oocytes of Xenopus laevis and by searching in transcriptomics databases. Candidate transporters will be validated in planta using loss-of-function mutants and grafting experiments with glucosinolate biosynthesis mutants and transporter mutants.

Ultimately, we expect to unravel how glucosinolates are translocated from production site to storage site, and to generate a dynamic model for movement of these compounds within and between organs in the plant throughout development.



Contact Persons

Barbara Ann Halkier
+45 353-33342

Deyang Xu

Scientific consultant
Deyang Xu
+45 353-33555